Application

Western blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1 unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10(7) units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D] .

Optimal working dilutions must be determined by end user.

Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 detects level of Tumor Necrosis Factor-β & has been published & validated for use in IC, IH & WB.

Immunogen

Human, recombinant tumor necrosis factor-beta (TNF-beta, lymphotoxin) produced in E. Coli.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified immunoglobulin (Protein A-agarose chormotography). One bottle of lyophilized preparation contains:200ug mouse IgG, 2 mg potassium phosphate buffer, 2.9 mg sodium chloride, and 20 mg raffinose.Reconstitution with 1 ml sterile, distilled water results in an antibody concentration of 200 ug/ml.

Format: Purified

Specificity

This antibody is use for detection, identification and neutralixzation of TNF-b in cell culture and other biological samples. The antibody reacts with human natural and recombinant TNF-b (lymphotoxin). It does not react with human TNF-a. Determination of cross-reactivity to TNF-b from other species has not been done.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.Freeze only once.